Immunohistochemistry and Immunofluorescence (IHCIF):

A Histochemical Society Hands-on Training Course

2023 Dates ANNOUNCED!

June 11-15, 2023 

Registration will open in early 2023.

2022 Course information below.  2023 updates will be posted periodically and before registration opens.

Read about the 2022 IHCIF Course and see a video preview of the program!

The Bioscience Education Center, Germantown, MD

This 4.5-day laboratory short course is ideal for experimental and translational research scientists, diagnosticians, and technical staff who are new to immunohistochemistry and immunofluorescence or those with experience who aim to take their skills to the next level. Our faculty are expert practitioners with diverse backgrounds in terms of the theory and application of these techniques as well as microscopy. Faculty to student ratios are low (1:3 or better) to maximize participants’ experience at the course and foster networking opportunities during and beyond the course. Lunch and dinner will be included.

Space limited to 24 participants

2022 Registration Fee: $1695.00

4.5 Day Training Workshop (45 contact hours)

Most meals included, housing NOT included.

Course Co-Directors: Charles Frevert, Professor at University of Washington and Paul Goodwin, Science Director at Cytiva

Biotrac Information and Registration Site:


  • Pre-analytics: Sample acquisition, fixation, processing embedding and microtomy
  • Reagents and validation: Reagents, antibody validation, protocol optimization and controls
  • Fundamentals of labels and stains: color, chromogen and fluorescent detection systems
  • Microscopy basics: transmitted light and fluorescence microscopes, limitations of the microscope and slide
  • Faculty facilitated discussions
  • Troubleshooting sessions
  • Advanced immunohistochemistry topics: antigen retrieval, multiplex immunohistochemistry, other detection systems and molecular targets
  • Horizon Lectures – automated IHC, quantitative digital pathology, digital imaging and deep learning, highly multiplexed IHC and TBA

Hands-on Laboratories:

  • Immunohistochemistry laboratory
  • Dual label immunofluorescence laboratory
  • Microscopy and digital imaging
  • Coverslipping

2022 Faculty:

Faculty to student ratios are low (1:3 or better.) The course offers a unique opportunity to learn from the best in the field and to continue collaborations after the event closes.  (2023 Faculty will be announced in early 2023.)

  Denis Baskin, PhD
Denis Baskin received his PhD from the University of California Berkeley and currently is an emeritus professor in the Departments of Medicine and Biological Structure at the University of Washington. He also is a retired Research Career Scientist for the Department of Veterans Affairs Medical Research Service. Dr Baskin’s research focused on the neuroendocrinology of food intake and body weight regulation, particularly related to diabetes and obesity. His research emphasized the development and application of immunohistochemical methods for the cellular localization and imaging of peptides and their receptors in the CNS, intestines, and pancreas. Dr Baskin is a past president of the Histochemical Society and he also served for 10 years as former editor-in-chief of the Journal of Histochemistry & Cytochemistry.
  A. Sally Davis DVM, PhD, DACVP, DACVM
Resident Education and Outreach Lead, The Histochemical Society, Rockville, MD
Adjunct Faculty, Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS
Extraordinary Faculty, Department of Paraclinical Sciences, Veterinary Faculty, University of Pretoria, Onderstepoort, Gauteng, South AfricaPhD

Sally Davis is a board-certified veterinary pathologist and microbiologist whose research focuses on emerging and zoonotic viral pathogens. She has 15 years of experience in the development of tissue-based assays, particularly immunohistochemistry, for a diverse array of species, tissue and target types. She is an educator who has taught both trainee and established researchers these techniques in her laboratory at Kansas State University.
  Francesca E. Duncan, PhD
Assistant Professor, Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University
Co-Director, Center for Reproductive Science, Northwestern University
Assistant Professor-in-Residence, Buck Institute for Research on Aging, Novato, CA

Francesca Duncan is a reproductive biologist whose research focuses on mechanisms of female reproductive aging. Given the complex tissue architecture and cellular heterogeneity of the mammalian ovary, histology and histochemical approaches are central to her research in multiple species including human, nonhuman primate, cow, pig, mouse, and naked mole rat. She is a proud alumna of the 2016 Immunhistochemical and Microscopy course which is the predecessor of the current course and now serves on the Histochemical Society Council.
  Charles W. Frevert, DVM, ScD
Professor, Department of Comparative Medicine, University of Washington (UW), Seattle, WA
Professor, Department of Medicine, Division of Pulmonary Critical Care Medicine and Sleep Medicine, UW
Adjunct Professor, Department of Pathology, UW

Charles Frevert, a veterinary scientist, and comparative pathologist, is a professor in the Department of Comparative Medicine at the University of Washington School of Medicine. In addition to his research, Dr. Frevert is the Director of the Histology and Imaging Core, a state-of-the-art research pathology laboratory at the University of Washington specializing in immunohistochemistry and quantitative digital pathology.
  Paul C. Goodwin, MS
Past-President, The Histochemical Society
Science Director, Cytiva, Issaquah, WA
Affiliate Teaching Associate, Department of Comparative Medicine, University of Washington, Seattle, WA

Paul Goodwin is the Science Director for Cytiva and resides in Seattle, WA. He explores the future of science, technology, and business models that will affect Life Sciences and investigate ways to convert challenges into business opportunities. He has been on the faculty for numerous microscopy courses at the Marine Biological Laboratory (MBL) in Woods Hole, MA, and Cold Spring Harbor Laboratories, Cold Spring Harbor, NY, for many years and he is the Past-President of The Histochemical Society.
  Madison Gowett
Graduate Student, Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University

Madison Gowett is a master’s student at Northwestern University in the Duncan Lab and her research focuses on ovarian aging. Specifically, she is interested in developing novel 3D culture approaches to study aging in the ovarian stromal compartment, and she utilizes various histochemical techniques to characterize this system.
  Stephen M. Hewitt. MD, PhD
Head, Experimental Pathology Laboratory, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD

Stephen Hewitt is Editor-in-Chief of the Journal of Histochemistry & Cytochemistry and co-chaired the Clinical Laboratory Standards Institute committee on guidelines for immunohistochemistry in clinical diagnostic laboratories.
  Leon Schermerhorn, DVM
Leon Schermerhorn is a licensed small animal veterinarian with a special interest in veterinary anatomic and experimental pathology. He has worked with Sally Davis on various projects including IHC protocol automation and primary cell culture of zoonotic mammalian pathogens. He particularly enjoys immunohistochemistry and exploring its uses in diagnostic and experimental settings.
  Scott Tanner, PhD
Assistant Professor of Biology, Division of Natural Sciences & Engineering University of South Carolina Upstate

Scott Tanner is an Assistant Professor at the University of South Carolina Upstate, where he focuses on undergraduate education. He was trained in the pathology laboratory of Dr. Robin Lorenz. Using C. elegans, his current research focuses on development of the intestinal barrier. He has experience using immunohistochemistry in a variety of tissue types.



The Histochemical Society is pleased to be partnering with BioTrac and Montgomery College for this course. The registration process is managed by Montgomery College. Registration will open in early 2023. You can find the BioTrac registration site here.

Travel Awards:

HCS will be offering awards to assist those who will travel to the course.  An affordable hotel option and free parking are available. But we understand travel costs add up.  Once you register for the course, out of town registrants may apply for an HCS travel award through the HCS travel award program.